
The multi-disciplinary efforts required in the scientific programme of
INTERACTION PROTEOME are organised into four sub-projects (SP):
SP1: Tools for interaction analysis
SP1 is dedicated to the development of innovative proteomics technology
to map protein-interaction networks and their cellular topology for the
interaction analyses in SP2 and SP3.
Workpackages in SP1:
WP1 Instrumentation for mass spectrometry
WP2 Gel-free technology for mass spectrometry
WP3 Parallel optical detection of protein interactions in living cells
WP4 Cryo Electron tomography
SP2: Identification of interaction
partners for protein domains
SP2 will generate (high throughput) data for important
protein-protein interactions defined by bioinformatics and biomedical
interest and by SP3, utilizing technology developed in SP1.
Workpackages in SP2:
WP5 Domain definition and expression
WP6 Interaction analysis in array format
WP7 Interaction analysis by bait fishing
WP8 In silico prediction of partners
WP9 Interpretation and database
SP3: Functional analysis of
interactions
SP3 focuses on the validation of technologies and tools developed in SP1.
It will perform functional analyses of protein-interactions in medically
and biochemically relevant prokaryotic and eukaryotic (mammalian) model
systems.
Work Packages in SP3:
WP10 Protein folding
WP11 Interaction mapping in cell signalling
SP4: Interactome database and
modelling
SP4 provides the required bioinformatics infrastructure for the project,
comprising the improvement of the public MINT database for the collection
and dissemination of the interactome data; modelling and simulation of
protein-interaction networks characterised in SP2 and SP3; and the dissemination
of the technology developments to the scientific community.
Work Packages in SP4:
WP12 MINT database
WP13 Modelling
WP14 Interface to Scientific Community
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