Abstract: 

In 1968, Roger G. Hart reported for the first time on the polytropic montage, which in nowadays terminology is addressed as electron tomography (ET) or three-dimensional electron microscopy (3DEM). Therefore, 3DEM is, by all means, not a new technique. However, some of the major technical and sample related difficulties had to be resolved over the last decades, directly leading to today’s success in the field of molecular structural biology. In the following paragraphs we will not focus on the technique itself, but on its applications in molecular structural biology and especially in its implications and promises for structural proteomics.

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